Providencia rettgeri as the causal agent of the brown slime flux of Populus tomentosa

Brown slime flux seeps slowly out of wounds and flows down the bark of roadside Populus tomentosa in Beijing, China. Two bacterial isolates, SL2‐2 and SL3‐3, obtained from the brown slime flux were identified as Providencia rettgeri based on 16S rRNA gene sequencing. After inoculation into the bark of roadside P. tomentosa with sterile deionized water as a control, both SL2‐2 and SL3‐3 triggered the seepage of brown slime flux from the wounds; no slime flux arose from the control. In conclusion, P. rettgeri was the pathogenic bacterium causing brown slime flux on P. tomentosa probably by inhibiting wound healing. To our knowledge, this is the first report of P. rettgeri as a pathogen of plants.     

紫草素抑制炎症促进驴皮伤口愈合的效果

试验旨在探究紫草素凝胶对驴伤口愈合的效果，为皮肤无瘢痕愈合提供参考。用不同浓度的紫草素对体外培养的驴巨噬细胞进行处理，分别检测细胞中TGF-β1、IL-10、TNF-α和IL-6的mRNA与细胞上清液中蛋白的表达水平，以确定紫草素的最佳添加浓度；建立驴皮伤口模型，在伤口表面涂抹紫草素凝胶，拍照、HE染色观察伤口愈合情况，并检测驴皮肤中伤口愈合相关基因和血清的表达水平。结果表明，与对照组相比，添加不同浓度的紫草素均能降低相关炎症因子的表达，其中10 μmol/L紫草素效果最佳。在驴皮伤口上涂抹紫草素凝胶第11天时，紫草素组的伤口收缩率极显著高于对照组（P<0.01）；HE染色发现，第11天时紫草素中的组织逐渐排列整齐；第60天时，紫草素组中表皮层的厚度相对减少；实时荧光定量PCR结果表明，第11天时，紫草素组中TGF-β1、IL-10 mRNA表达极显著升高（P<0.01），TNF-α、IL-6 mRNA的表达极显著降低（P<0.01）；第3天时，紫草素组血清中TGF-β1、IL-10的浓度升高，TNF-α、IL-6浓度极显著降低（P<0.01）；第7天时，紫草素组TGF-β1浓度显著降低（P<0.05），TNF-α浓度极显著降低（P<0.01），且IL-10的浓度显著升高（P<0.05）。综上，与对照组相比，添加不同浓度的紫草素均能降低相关炎症因子的表达，其中10 μmol/L紫草素效果最佳，能促进驴皮伤口的愈合。     

湿地松割面全愈合采脂技术的研究

经过5 a的试验,通过采用控制中沟的深度不深入木质部,割面负荷率不超过60%,每年的下降幅度不超过12 cm,每年起割时在距上年最后一次割沟处设置1～2愈合带的方法,可使每年采脂产生的割面在2 a的时间内完全愈合,以增强采脂树的抗折断力及消除割面因无树皮保护而造成的腐朽现象并提高采脂树的养分输送能力,进而提高树木的产脂量。     

苹果轮纹病室内快速评价体系的建立

为探索高效、稳定且操作简便的苹果轮纹病室内评价方法,选用分离菌株Slg08O3-1-1-1菌饼分别对离体富士苹果两年生枝条以及当年生嫩梢、叶片和成熟果实4种材料进行接种,并对试验结果和接种方法进行比较分析.结果显示,在有伤和无伤条件下各接种材料接种无菌PDA块均不发病,接种病菌仅在有伤情况下致病;枝条烫伤接种发病最慢且病斑最小,嫩梢针刺1针接种较叶痕接种发病明显;1针及10针刺伤接种叶片正、反面,正面1针刺伤接种发病较快且一致;果实去除果皮接种较1针、10针刺伤后接种发病显著.用4个致病力不同的菌株验证,发现4种离体材料均能验证不同菌株间的致病力差异,且结果与田间无伤接种当年生新梢一致.说明4种离体材料均可准确、快速评价苹果轮纹病,其中正面针刺1针接种叶片的方法最优.     

双断根嫁接对辣椒嫁接苗愈合及生长的影响

以"巨根"辣椒为砧木,"龙椒六号"辣椒为接穗,以砧木不断根常规嫁接和辣椒自根苗分别作为对照,研究双断根套管嫁接方法对辣椒嫁接苗愈合及苗期生长的影响,以期为辣椒种植提供参考依据。结果表明:双断根套管嫁接可有效预防辣椒嫁接苗的徒长,显著提高壮苗指数。辣椒双断根套管嫁接时,砧木与接穗结合部的伤口愈合与砧木根系再生同时进行。嫁接可以有效提高根系活力,采取双断根套管嫁接后根系活力的提升更显著,根系活力随着嫁接时间的延长呈现降低趋势,双断根套管嫁接始终显著高于常规嫁接苗。2种嫁接方法之间的输导组织运输能力差异不显著;与自根苗相比,2种嫁接方法的输导能力在结合部愈合前期显著降低,在结合部完全愈合、嫁接苗成活后(嫁接后20 d)输导能力显著提高,且双断根方法略高于常规嫁接。     

Differential analysis of serum proteomics in Crohn's disease treated with infliximab

AIM: To identify the serum proteins that might serve as biomarkers for predicting mucosal healing (MH) in the patients with Crohn's disease (CD) treated with infliximab (IFX). METHODS: We collected serum samples before treatment (0 week, group A) and 14 weeks after treatment (group B) from 7 CD patients with IFX treatment who had achieved MH, as well as the serum samples from 7 CD patients who had not achieved MH (0 week, group C; 14 weeks, group D). Two-dimensional fluorescence difference gel electrophoresis was applied to analyze and compare the results of serum profiles between groups A and B, C and D, A and C, B and D. Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and bioinformatics tools were utilized to preliminarily identify and figure out the differentially expressed proteins. RESULTS: (1) In total, there were 44 differentially expressed spots, 36, 3, 10 and 31 differentially expressed spots were detected while comparing A with B, C with D, A with C and B with D, respectively. (2) Among those spots, 17, 2, 2 and 15 proteins were identified, respectively. In total, there were 19 differentially expressed proteins, including apolipoprotein E, apolipoprotein A-I, complement factor H, and so on. (3) Protein functional association networks were carried out based on STRING database. CONCLUSION: The serum protein profiles obviously change after IFX treatment in MH CD patients, and the serum protein profiles of MH patients are different from that of non-MH patients after IFX treatment. The 19 proteins we identified may serve as potential biomarkers for predicting MH in CD patients with IFX treatment.     

药用植物红芽大戟研究概况

综述了珍贵药用植物红芽大戟的地理分布和生态环境、植物学形态、药用特性与药理作用、繁殖与栽培管理,及市场需求与展望。     

A cross‐linked hyaluronan gel accelerates healing of corneal epithelial abrasion and alkali burn injuries in rabbits

Objective To evaluate the efficacy of a chemically modified and cross‐linked derivative of hyaluronan (CMHA‐SX) for treatment of corneal epithelial abrasion and standardized alkali burn injuries. Animals Twelve female New Zealand white rabbits in two groups were used. Procedures Bilateral 6‐mm diameter corneal epithelial abrasions were made in each of six rabbits in one group and 6‐mm standardized alkali burn injuries were made in the second group. A 1% CMHA‐SX formulation was applied topically four times per day in right eye of each rabbit for 1 week, and phosphate buffered saline (PBS) was placed in left (control) eye of each rabbit. The wound size was determined by staining with 1% fluorescein and photographed at the slit lamp with a digital camera at 0, 1, 2, 3 days postoperatively in the first group and 0, 1, 2, 3, 7, 12 days in the second group. Rabbit corneas were collected for histological examination on day 7 in the first group and day 12 in the second group. Results Closure of corneal wound in the abrasion model was complete in the CMHA‐SX treated eye by 48 h. The wound closure rate and thickness of the central corneal epithelium in the CMHA‐SX treated group was greater than in control eyes for both the abrasion and alkali burn injuries. Moreover, the CMHA‐SX treated cornea exhibited better epithelial and stromal organization than the untreated control cornea. Conclusions Chemically modified and cross‐linked derivative of hyaluronan improved corneal wound healing and could be useful for treating noninfectious corneal injuries.     

Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets

This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg^?1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg^?1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound‐healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken‐line analysis showed that the supplementation of 148 mg organic Zn kg^?1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles.